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GDNF Receptor alpha 1/GFRA1 Recombinant Rabbit mAb (S-3289-52)

GDNF Receptor alpha 1/GFRA1 Recombinant Rabbit mAb (S-3289-52)

Catalog Number: S0B6580 Application: WB, IHC-P Reactivity: Human Conjugation: Unconjugated Brand: Starter
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Product Details

Product Specification


Host Rabbit
Antigen GDNF Receptor alpha 1/GFRA1
Synonyms GDNF family receptor alpha-1; GDNF receptor alpha-1; GDNFR-alpha-1; GFR-alpha-1; RET ligand 1; TGF-beta-related neurotrophic factor receptor 1; GDNFRA; RETL1; TRNR1
Immunogen Synthetic Peptide
Location Endosome, Cell membrane
Accession P56159
Clone Number S-3289-52
Antibody Type Recombinant mAb
Isotype IgG
Application WB, IHC-P
Reactivity Hu
Positive Sample MCF7
Purification Protein A
Concentration 0.5 mg/ml
Conjugation Unconjugated
Physical Appearance Liquid
Storage Buffer

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
Stability & Storage

12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution


application dilution species
WB 1:1000 Hu
IHC-P 1:1000 Hu

Background

GDNF family receptor alpha-1 (GFRA1) is a 465-amino-acid, glycosylphosphatidylinositol-anchored cell-surface glycoprotein that functions as the high-affinity ligand-binding component for glial-cell-line-derived neurotrophic factor (GDNF) and its close homolog neurturin; upon ligand binding GFRA1 nucleates formation of a tripartite complex that recruits and trans-activates the transmembrane tyrosine kinase RET, thereby triggering downstream survival, proliferation and neurite-outgrowth signals (PI3K/AKT, RAS/MAPK, PLCγ) in dopaminergic, motor, sensory and enteric neurons, and this same GFRA1-mediated signaling is essential during kidney morphogenesis, spermatogenesis and peripheral-nerve repair while also being implicated in cancer chemoresistance and Parkinson’s disease.

Picture

Western Blot

WB result of GDNF Receptor alpha 1/GFRA1 Recombinant Rabbit mAb
Primary antibody: GDNF Receptor alpha 1/GFRA1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-3 whole cell lysate 20 µg
Lane 2: MCF7 whole cell lysate 20 µg
Negative control: PC-3 whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 51 kDa
Observed MW: 47, 60 kDa
This blot was developed with high sensitivity substrate

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-GDNF Receptor alpha 1/GFRA1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human kidney. Anti-GDNF Receptor alpha 1/GFRA1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human breast cancer. Anti-GDNF Receptor alpha 1/GFRA1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.