2μg (R: reducing condition, N: non-reducing condition).
GDF-15/MIC-1 His Tag Protein, Cynomolgus
GDF-15/MIC-1 His Tag Protein, Cynomolgus
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$215 USD
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Product Details
Product Details
Product Specification
| Species | Cynomolgus |
| Synonyms | MIC1, NAG-1, PDF, PLAB, PTGFB, NRG-1 |
| Accession | G7PWZ3 |
| Amino Acid Sequence | Ala197-Val 308 with His Tag at the N-Terminus |
| Expression System | HEK293 |
| Molecular Weight | 10-15kDa (Reducing) |
| Purity | ＞95% by SDS-PAGE |
| Conjugation | Unconjugated |
| Tag | His Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | 20 mM Citric acid, 200 mM NaCl, pH2.2, 5% trehalose |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage |
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
| Reference | 1. Emmerson, P.J., et al. (2017). GFRAL is the receptor for GDF15 and is required for the anti-obesity effects of the ligand. Nature Medicine, 23(10), 1158-1166. |
Background
GDF15 (Growth Differentiation Factor 15) is a divergent member of the Transforming Growth Factor-β (TGF-β) superfamily. Initially identified in activated macrophages, it is a stress-induced cytokine whose expression is upregulated under conditions such as tissue hypoxia, inflammation, acute injury, and oxidative stress. GDF15 is highly expressed in the placenta and prostate and acts as a pleiotropic cytokine involved in the cellular stress response program.
Functionally, GDF15 signals through its specific receptor, GFRAL (GDNF Family Receptor Alpha Like), which is primarily expressed in the area postrema and nucleus tractus solitarius of the brainstem. Upon binding, GDF15 recruits the co-receptor RET to initiate signaling. This axis constitutes an "emergency circuit" that regulates feeding responses to metabolic and toxin-induced stresses, modulating food intake, energy expenditure, and body weight. GDF15 also promotes nausea and vomiting, with elevated levels linked to pregnancy-induced morning sickness and hyperemesis gravidarum.
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SDS-PAGE
ELISA
Immobilized GDF-15 / MIC-1 His Tag Protein, Cynomolgus (Cat.No.UA016200) at 1 μg/mL (100 μL/well) can bind GFRAL Fc Chimera Protein, Human (Cat.No. UA011250) with a linear range of 1.47-2.55 ng/mL (QC tested).
