WB result of GCC1 Recombinant Rabbit mAb
Primary antibody: GCC1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: A431 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: HEK-193 whole cell lysate 20 µg
Lane 4: HepG2 whole cell lysate 20 µg
Lane 5: SK-BR-3 whole cell lysate 20 µg
Lane 6: SH-SY5Y whole cell lysate 20 µg
Lane 7: Ramos whole cell lysate 20 µg
Lane 8: HT-29 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 88 kDa
Observed MW: 105 kDa
GCC1 Recombinant Rabbit mAb (S-4449-71)
GCC1 Recombinant Rabbit mAb (S-4449-71)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | GCC1 |
| Synonyms | GRIP and coiled-coil domain-containing protein 1 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm |
| Accession | Q96CN9 |
| Clone Number | S-4449-71 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | A431, HeLa, HEK-293, HepG2, SK-BR-3, SH-SY5Y, Ramos, HT-29, mouse ovary, rat ovary |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
Background
GCC1, also known as GRIP and coiled-coil domain-containing protein 1 or GCC185, is a large peripheral membrane protein localized primarily to the trans-Golgi network (TGN) that functions as an essential tethering factor in retrograde transport by anchoring Rab9-positive vesicles carrying mannose-6-phosphate receptors from late endosomes back to the TGN. Structurally characterized by extensive coiled-coil domains and multiple Rab-binding sites, GCC1 interacts not only with active Rab9 GTPase but also with SNARE proteins such as syntaxin 6 and syntaxin 16, thereby facilitating vesicle docking and fusion while maintaining Golgi architecture and ensuring proper lysosomal enzyme sorting; consequently, dysfunction or depletion of GCC1 disrupts endosome-to-TGN trafficking, leading to missorting of cargo, fragmented Golgi morphology, and impaired cellular homeostasis, highlighting its critical role in intracellular logistics and organelle integrity.
Picture
Picture
Western Blot
WB result of GCC1 Recombinant Rabbit mAb
Primary antibody: GCC1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse ovary lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 88 kDa
Observed MW: 105 kDa
WB result of GCC1 Recombinant Rabbit mAb
Primary antibody: GCC1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat ovary lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 88 kDa
Observed MW: 105 kDa
