WB result of GBP2 Recombinant Rabbit mAb
Primary antibody: GBP2 Recombinant Rabbit mAb at 1/10000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HaCaT whole cell lysate 20 µg
Lane 3: K-562 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 67 kDa
Observed MW: 67 kDa
GBP2 Recombinant Rabbit mAb
GBP2 Recombinant Rabbit mAb
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$100 USD
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | GBP2 |
| Synonyms | Guanylate-binding protein 2; GTP-binding protein 2 (GBP-2; HuGBP-2); Guanine nucleotide-binding protein 2; Interferon-induced guanylate-binding protein 2 |
| Location | Cytoplasm |
| Accession | P32456 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu |
| Positive Sample | HeLa, HaCaT, K-562 |
| Purification | Protein A |
| Concentration | 1 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:2000-1:20000 | Hu |
| IHC-P | 1: 200-1:800 | Hu |
Background
Guanylate-binding protein 2 (GBP2) is a core component of the innate immune system, induced by interferons (IFNs) and belonging to the dynamin-related GTPase family. It binds and hydrolyzes GTP, with GDP being its primary hydrolysis product. Functionally, GBP2 serves as a critical effector molecule against a broad spectrum of bacterial, viral, and protozoan pathogens. Its mechanisms of action include: on one hand, it can rupture pathogen-containing vacuoles, releasing pathogens into the cytosol and thereby activating the inflammasome signaling pathway to trigger robust immune responses; on the other hand, it can exert antiviral effects independently of its GTPase activity by inhibiting the maturation of viral envelope proteins. Structurally, GBP2 possesses a globular G domain and a flexible C-terminal helical domain. Its GTPase activity is tightly regulated, and it undergoes dynamic interconversion between monomeric and dimeric states during the GTP hydrolysis cycle. These sophisticated structural and functional features position GBP2 as a key player in cell-autonomous immunity.
Picture
Picture
Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human spleen. Anti-GBP2 antibody was used at 1/400 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti-GBP2 antibody was used at 1/400 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-GBP2 antibody was used at 1/400 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
