WB result of GBA Recombinant Rabbit mAb
Primary antibody: GBA Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: SH-SY5Y whole cell lysate 20 µg
Lane 2: U-87 MG whole cell lysate 20 µg
Lane 3: MCF7 whole cell lysate 20 µg
Lane 4: 293T whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 60 kDa
Observed MW: 60 kDa
GBA Recombinant Rabbit mAb (S-3211-14)
GBA Recombinant Rabbit mAb (S-3211-14)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | GBA |
| Synonyms | Lysosomal acid glucosylceramidase; Lysosomal acid GCase; Acid beta-glucosidase; Alglucerase; Beta-glucocerebrosidase (Beta-GC); Beta-glucosylceramidase 1; Cholesterol glucosyltransferase (SGTase); GC; GLUC; GBA1 |
| Immunogen | Synthetic Peptide |
| Location | Lysosome |
| Accession | P04062 |
| Clone Number | S-3211-14 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Mk |
| Positive Sample | SH-SY5Y, U-87 MG, MCF7, 293T |
| Predicted Reactivity | Pg, Cz, Or |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Mk |
| IHC-P | 1:1000 | Hu |
Background
GBA protein, encoded by the GBA gene on chromosome 1q21, is a 497-amino-acid lysosomal glucocerebrosidase that catalyzes the hydrolysis of glucosylceramide to glucose and ceramide; homozygous mutations cause Gaucher disease, while heterozygous mutations are the strongest common genetic risk factor for Parkinson’s disease and dementia with Lewy bodies through gain-of-function mechanisms that promote α-synuclein aggregation, impaired autophagy-lysosomal flux, and neuroinflammation, and its enzymatic activity can be enhanced by small-molecule chaperones or gene therapy, making it a key therapeutic target for both systemic and neurodegenerative disorders.
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Picture
Western Blot
WB result of GBA Recombinant Rabbit mAb
Primary antibody: GBA Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 60 kDa
Observed MW: 60 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human colon. Anti-GBA antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-GBA antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti-GBA antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
