GATA1 Recombinant Rabbit mAb (S-5401)

Catalog Number: S0B60356 Application: WB, IHC-P, ICC Reactivity: Hu, Ms, Rt Conjugation: Unconjugated Brand: Starter

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$100 USD

Size: 25μl 100μl

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Product Details

Product Specification

Host	Rabbit
Antigen	GATA1
Synonyms	Erythroid transcription factor; Eryf1; GATA-binding factor 1 (GATA-1; GF-1); NF-E1 DNA-binding protein; GF1; GATA1
Location	Nucleus
Accession	P15976
Clone Number	S-5401
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P, ICC
Reactivity	Hu, Ms, Rt
Positive Sample	K-562
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000	Hu, Ms, Rt
IHC-P	1:500	Hu, Ms, Rt
ICC	1:500	Hu

Background

GATA1 is a zinc finger transcription factor that serves as the master regulator of erythroid and megakaryocytic differentiation, playing an indispensable role in hematopoiesis by binding to the consensus DNA sequence (A/T)GATA(A/G) to activate genes essential for red blood cell maturation, such as those encoding globins and heme biosynthesis enzymes, while simultaneously repressing proliferation-associated genes to facilitate terminal differentiation. Its activity is tightly controlled through post-translational modifications and critical protein-protein interactions with cofactors like FOG-1 (ZNF423), which modulates its transcriptional output, and mutations in the GATA1 gene are clinically significant as they cause X-linked dyserythropoietic anemia, thrombocytopenia, and are strongly associated with transient abnormal myelopoiesis and acute megakaryoblastic leukemia in individuals with Down syndrome, underscoring its vital function in normal blood development and its pathological consequences when dysregulated.

Picture

Western Blot

WB result of GATA1 Recombinant Rabbit mAb
Primary antibody: GATA1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: K-562 whole cell lysate 20 µg
Negative control: HeLa whole cell lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L) HRP conjugated at 1/10000 dilution
Predicted MW: 43 kDa
Observed MW: 43 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human colon cancer. Anti-GATA1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human lung cancer. Anti-GATA1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse bone marrow. Anti-GATA1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat spleen. Anti-GATA1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Immunocytochemistry

ICC shows positive staining in K562 cells (top panel) and negative staining in HeLa cells (below panel). Anti-GATA1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).