WB result of GAP43 Rabbit pAb-Neuronal Marker
Primary antibody: GAP43 Rabbit pAb-Neuronal Marker at 1/1000 dilution
Lane 1: SH-SY5Y whole cell lysate 20 µg
Lane 2: IMR-32 whole cell lysate 20 µg
Lane 3: TT whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 25 kDa
Observed MW: 45-50 kDa
GAP43 Rabbit Polyclonal Antibody-Neuronal Marker
GAP43 Rabbit Polyclonal Antibody-Neuronal Marker
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | GAP43 |
| Synonyms | Neuromodulin; Axonal membrane protein GAP-43; Growth-associated protein 43; Neural phosphoprotein B-50; pp46 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Cell membrane, Synapse |
| Accession | P17677 |
| Antibody Type | Polyclonal antibody |
| Isotype | IgG |
| Application | WB, IHC-P, IF |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | SH-SY5Y, IMR-32, TT, Neuro-2a, mouse brain, PC-12, rat brain |
| Predicted Reactivity | CyMk |
| Purification | Immunogen Affinity |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| IHC-P | 1:1000 | Hu, Ms, Rt |
| IF | 1:1000 | Ms |
Background
GAP43 protein, also known as growth-associated protein 43, is a crucial molecule in the nervous system. It is predominantly expressed in neurons and plays a vital role in axonal growth and regeneration. During the development of the nervous system, GAP43 is highly expressed in growth cones at the tips of growing axons, where it helps to mediate the interactions between the axon and its surrounding environment, guiding the direction of axonal extension. In the adult nervous system, its expression can be upregulated in response to injury, facilitating the repair and reconnection of damaged neural circuits. Additionally, GAP43 is involved in synaptic plasticity, contributing to the strengthening and remodeling of synapses, which is essential for learning and memory processes. Its phosphorylation and dephosphorylation states can regulate its function, and alterations in GAP43 levels or activity have been implicated in various neurological disorders, making it an important target for research in neuroregeneration and neurodegenerative diseases.
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Western Blot
WB result of GAP43 Rabbit pAb-Neuronal Marker
Primary antibody: GAP43 Rabbit pAb-Neuronal Marker at 1/1000 dilution
Lane 1: Neuro-2a whole cell lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 25 kDa
Observed MW: 45-55 kDa
WB result of GAP43 Rabbit pAb-Neuronal Marker
Primary antibody: GAP43 Rabbit pAb-Neuronal Marker at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: rat brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 25 kDa
Observed MW: 45-57 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-GAP43 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human cerebral cortex. Anti-GAP43 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-GAP43 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-GAP43 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunofluorescence
IF shows positive staining in paraffin-embedded mouse hippocampus. Anti-GAP43 antibody was used at 1/1000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
