WB result of GADD34 Recombinant Rabbit mAb
Primary antibody: GADD34 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HepG2 whole cell lysate 20 µg
Lane 2: Jurkat whole cell lysate 20 µg
Lane 3: K562 whole cell lysate 20 µg
Lane 4: PC-3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 73 kDa
Observed MW: 120 kDa
This blot was developed with high sensitivity substrate
GADD34 Recombinant Rabbit mAb (S-2267-246)
GADD34 Recombinant Rabbit mAb (S-2267-246)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | GADD34 |
| Synonyms | Protein phosphatase 1 regulatory subunit 15A; Growth arrest and DNA damage-inducible protein GADD34; Myeloid differentiation primary response protein MyD116 homolog; PPP1R15A |
| Immunogen | Synthetic Peptide |
| Location | Mitochondrion, Endoplasmic reticulum |
| Accession | O75807 |
| Clone Number | S-2267-246 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Mk |
| Positive Sample | HepG2, Jurkat, K562, PC-3, COS-7 |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Mk |
Background
GADD34, also known as PPP1R15A, is a stress-inducible regulatory subunit of protein phosphatase 1 (PP1) that plays a crucial role in cellular stress responses by forming a complex with PP1 to dephosphorylate eIF2α, thereby promoting translational recovery during the integrated stress response (ISR), and is involved in various cellular processes including apoptosis, cell cycle arrest, and DNA damage response, with its expression being upregulated by a variety of stress signals such as DNA damage, endoplasmic reticulum (ER) stress, and oxidative stress, and has been implicated in several pathological conditions including cancer, neurodegenerative diseases, and metabolic disorders.
Picture
Picture
Western Blot
WB result of GADD34 Recombinant Rabbit mAb
Primary antibody: GADD34 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 73 kDa
Observed MW: 120 kDa
This blot was developed with high sensitivity substrate
