GAB1 Recombinant Rabbit mAb (S-3907-33)

Catalog Number: S0B60396 Application: WB, IHC-P Reactivity: Hu, Ms, Mk Conjugation: Unconjugated Brand: Starter

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$100 USD

Size: 25μl 100μl

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Product Details

Product Specification

Host	Rabbit
Antigen	GAB1
Synonyms	GRB2-associated-binding protein 1; GRB2-associated binder 1; Growth factor receptor bound protein 2-associated protein 1
Immunogen	Synthetic Peptide
Location	Cytoplasm
Accession	Q13480
Clone Number	S-3907-33
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P
Reactivity	Hu, Ms, Mk
Positive Sample	Ramos, HepG2, NIH/3T3, 3T3/L1, mouse brain, COS7
Predicted Reactivity	Bv, Hm
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000	Hu, Ms, Mk
IHC-P	1:250	Hu

Background

GAB1 is a core member of the Gab family of adaptor proteins, consisting of 694 amino acids with a molecular weight of approximately 77 kDa. As a multifunctional adapter protein, GAB1 lacks catalytic activity but serves as a critical bridging molecule in intracellular signal transduction through its N-terminal pleckstrin homology (PH) domain, proline-rich regions, and multiple tyrosine residues that can be phosphorylated. It can be activated by various receptor tyrosine kinases such as the epidermal growth factor receptor (EGFR) and the hepatocyte growth factor receptor (c-Met), and by binding to effector molecules including GRB2, the p85 subunit of PI3K, and the phosphatase SHP2, it mediates the activation of downstream signaling pathways such as PI3K-AKT and MAPK, thereby broadly participating in the regulation of cell growth, proliferation, migration, and apoptosis. Notably, GAB1 can directly bind to the c-Met receptor through its unique Met-binding domain (MBD), a feature that renders it an irreplaceable role in mediating hepatocyte growth factor (HGF)-induced cell branching morphogenesis and angiogenesis. Furthermore, functional aberrations or altered expression of GAB1 have been implicated in various diseases, including tumor development and progression, inflammatory responses, and hereditary non-syndromic deafness.

Picture

Western Blot

WB result of GAB1 Recombinant Rabbit mAb
Primary antibody: GAB1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Ramos whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 77 kDa
Observed MW: 100 kDa
This blot was developed with high sensitivity substrate

WB result of GAB1 Recombinant Rabbit mAb
Primary antibody: GAB1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: 3T3/L1 whole cell lysate 20 µg
Lane 3: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 77 kDa
Observed MW: 100 kDa

WB result of GAB1 Recombinant Rabbit mAb
Primary antibody: GAB1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 77 kDa
Observed MW: 100 kDa
This blot was developed with high sensitivity substrate

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human kidney. Anti-GAB1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human placenta. Anti-GAB1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human breast cancer. Anti-GAB1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human lung cancer. Anti-GAB1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.