ScFv based CAR containing a G4S linker transiently transfected T cells (from human PBMCs) (right panel) or T cells (left panel) were stained with Pacific Blue Rabbit Anti-G4S Linker Antibody at 5 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
G4S Linker Recombinant Rabbit mAb (Pacific Blue Conjugate) (S-5030)
G4S Linker Recombinant Rabbit mAb (Pacific Blue Conjugate) (S-5030)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | G4S Linker |
| Clone Number | S-5030 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Isotype Control | S0B1550 |
| Application | FCM |
| Reactivity | Species Independent |
| Purification | Protein A |
| Concentration | 0.2 mg/ml |
| Conjugation | Pacific Blue |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.09% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Species independent |
Background
G4S Linker is a commonly used flexible amino acid linker that plays a significant role in antibody expression and design. The sequence of this linker is (Gly-Gly-Gly-Gly-Ser)n, where "n" represents the number of repetitions. By altering the value of "n," the length of the GS amino acid chain can be optimized to achieve appropriate separation of antibody or protein domain functions or to maintain necessary inter-domain interactions. G4S Linker is typically used in single-chain variable fragments (scFv) and chimeric antigen receptors (CARs) to connect the variable heavy (VH) and variable light (VL) domains for the recognition of target antigens. The length of this linker is crucial for controlling the stability of scFv and the non-covalent association between the VH and VL domains. In CAR structures, the G4S Linker is located in the extracellular domain of the scFv, helping to maintain the structure and function of the CAR. Additionally, G4S Linker is used to detect CAR cells designed with G4S Linker, which offers high specificity and sensitivity.
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FC
ScFv based CAR containing a G4S linker transfected NK cells (from human PBMCs) (right panel) or NK cells (left panel) were stained with Pacific Blue Rabbit Anti-G4S Linker Antibody at 5 μl/test. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
