WB result of G3BP2 Recombinant Rabbit mAb
Primary antibody: G3BP2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: 293T whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: Jurkat whole cell lysate 20 µg
Lane 4: A549 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 54 kDa
Observed MW: 80 kDa
This blot was developed with high sensitivity substrate
G3BP2 Recombinant Rabbit mAb (S-2386-66)
G3BP2 Recombinant Rabbit mAb (S-2386-66)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | G3BP2 |
| Synonyms | Ras GTPase-activating protein-binding protein 2; G3BP-2; GAP SH3 domain-binding protein 2; KIAA0660 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm |
| Accession | Q9UN86 |
| Clone Number | S-2386-66 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Ms, Rt, Mk |
| Positive Sample | 293T, HeLa, Jurkat, A549, C2C12, mouse brain, C6, rat brain, COS-7 |
| Predicted Reactivity | Or |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt, Mk |
Background
G3BP2 (Ras-GTPase-activating protein-binding protein 2) is a multifunctional RNA-binding scaffold protein that, through its NTF2, acidic, proline-rich, RRM and RGG domains, nucleates stress-granule assembly by forming homo- and hetero-oligomers with G3BP1, sequestering untranslated mRNPs in the cytoplasm during stress, while also regulating RNA stability (e.g., CDK7, CDK9, c-Myc), controlling nuclear-cytoplasmic shuttling of transcription factors such as NF-κB, TWIST1 and p53, and thereby influencing cell proliferation, epithelial-mesenchymal transition, viral infection, lipogenesis and neuronal viability in a context-dependent manner.
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Picture
Western Blot
WB result of G3BP2 Recombinant Rabbit mAb
Primary antibody: G3BP2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C2C12 whole cell lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 54 kDa
Observed MW: 80 kDa
This blot was developed with high sensitivity substrate
WB result of G3BP2 Recombinant Rabbit mAb
Primary antibody: G3BP2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: rat brain lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 54 kDa
Observed MW: 80 kDa
This blot was developed with high sensitivity substrate
WB result of G3BP2 Recombinant Rabbit mAb
Primary antibody: G3BP2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 54 kDa
Observed MW: 80 kDa
This blot was developed with high sensitivity substrate
