WB result of FUNDC1 Rabbit pAb
Primary antibody: FUNDC1 Rabbit pAb at 1/1000 dilution
Lane 1: THP-1 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: T-47D whole cell lysate 20 µg
Lane 4: MCF7 whole cell lysate 20 µg
Negative control: THP-1 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 17 kDa
Observed MW: 17 kDa
FUNDC1 Rabbit Polyclonal Antibody
FUNDC1 Rabbit Polyclonal Antibody
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | FUNDC1 |
| Synonyms | FUN14 domain-containing protein 1 |
| Immunogen | Synthetic Peptide |
| Location | Mitochondrion |
| Accession | Q8IVP5 |
| Antibody Type | Polyclonal antibody |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HeLa, T-47D, MCF7, RAW264.7, mouse brain, C6, rat brain |
| Purification | Immunogen Affinity |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:500-1:1000 | Hu, Ms, Rt |
| IHC-P | 1:500 | Hu, Ms, Rt |
Background
FUNDC1 (FUN14 domain containing 1) is a protein located in the outer mitochondrial membrane and mitochondrial-associated membranes (MAMs), playing a crucial role in regulating mitochondrial quality control through mitophagy, mitochondrial dynamics, and biogenesis. It contains an LC3 interaction region (LIR) motif that binds to LC3, facilitating the selective degradation of damaged mitochondria to maintain cellular health. Under hypoxic conditions, FUNDC1 is phosphorylated by ULK1 and dephosphorylated by PGAM5, enhancing its interaction with LC3 and promoting mitophagy. Additionally, FUNDC1 collaborates with OPA1 to modulate mitochondrial dynamics, and its phosphorylation state affects mitochondrial morphology and the balance between fusion and fission. It is extensively expressed in the heart and has been implicated in various diseases, including metabolic cardiomyopathy, cardiac remodeling, and myocardial ischemia-reperfusion injury.
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Western Blot
WB result of FUNDC1 Rabbit pAb
Primary antibody: FUNDC1 Rabbit pAb at 1/1000 dilution
Lane 1: RAW264.7 whole cell lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 17 kDa
Observed MW: 17 kDa
WB result of FUNDC1 Rabbit pAb
Primary antibody: FUNDC1 Rabbit pAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: rat brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 17 kDa
Observed MW: 17 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-FUNDC1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human pancreatic cancer. Anti-FUNDC1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-FUNDC1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cardiac muscle. Anti-FUNDC1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
