WB result of FOXP4 Recombinant Rabbit mAb
Primary antibody: FOXP4 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 μg
Lane 2: HepG2 whole cell lysate 20 μg
Lane 3: Caco-2 whole cell lysate 20 μg
Lane 4: COLO 205 whole cell lysate 20 μg
Lane 5: RD whole cell lysate 20 μg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 73 kDa
Observed MW: 85 kDa
FOXP4 Recombinant Rabbit mAb (S-6198)
FOXP4 Recombinant Rabbit mAb (S-6198)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | FOXP4 |
| Synonyms | Forkhead box protein P4; FKHLA |
| Location | Nucleus |
| Accession | Q8IVH2 |
| Clone Number | S-6198 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HeLa, HepG2, Caco-2, COLO 205, 4T1, PC-12 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:5000 | Hu, Ms, Rt |
| IHC-P | 1:2000 | Hu, Ms, Rt |
| ICC | 1:500 | Hu |
Background
FOXP4 (Forkhead box protein P4) is a functionally critical transcription factor belonging to the FOXP subfamily, predominantly exerting transcriptional repressive functions within the nucleus. Its activity depends on forming homodimers via its leucine zipper domain or forming heterodimers with FOXP1 and FOXP2 to bind DNA. In physiological development, it is a multifunctional regulator essential for the morphogenesis of organs such as the heart, lung, and intestine, playing critical roles in embryonic foregut morphogenesis, pulmonary secretory cell differentiation, and the maintenance of cerebellar Purkinje cell dendrites. However, dysregulation of FOXP4 is closely associated with various diseases: heterozygous mutations or homozygous loss-of-function variants have been confirmed to cause autosomal dominant or recessive genetic disorders, with patients typically presenting with short stature, developmental malformations, neurodevelopmental disorders, congenital heart disease, and language developmental delay among a spectrum of congenital abnormalities. Additionally, FOXP4 plays a complex "dual-faceted" role in tumorigenesis—it may act as a tumor suppressor downregulated in renal cancer, but conversely functions as an oncogene overexpressed in non-small cell lung cancer, hepatocellular carcinoma, and prostate cancer, where it promotes tumor growth and invasion.
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Western Blot
WB result of FOXP4 Recombinant Rabbit mAb
Primary antibody: FOXP4 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: 4T1 whole cell lysate 20 μg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 73 kDa
Observed MW: 85 kDa
WB result of FOXP4 Recombinant Rabbit mAb
Primary antibody: FOXP4 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 μg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 73 kDa
Observed MW: 85 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human colon. Anti-FOXP4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti-FOXP4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-FOXP4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-FOXP4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-FOXP4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse spleen. Anti-FOXP4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat spleen. Anti-FOXP4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat stomach. Anti-FOXP4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in HepG2 cells. Anti-FOXP4 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
