WB result of FOXP3 Recombinant Mouse mAb
Primary antibody: FOXP3 Recombinant Mouse mAb at 1/1000 dilution
Lane 1: mouse heart lysate 20 µg
Secondary antibody: Goat Anti-mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 47 kDa
Observed MW: 50 kDa
FOXP3 Recombinant Mouse mAb (SDT-3494)
FOXP3 Recombinant Mouse mAb (SDT-3494)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | FOXP3 |
| Synonyms | Forkhead box protein P3; Scurfin; IPEX |
| Location | Cytoplasm, Nucleus |
| Accession | Q9BZS1 |
| Clone Number | SDT-3494 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1,k |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | mouse heart |
| Purification | Protein G |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Ms |
| IHC-P | 1:250-1:1000 | Hu, Ms, Rt |
Background
FOXP3 protein is a crucial transcription factor that plays a central role in the development and function of regulatory T cells (Tregs), which are essential for maintaining immune tolerance and preventing autoimmune diseases. It is predominantly expressed in Tregs and helps suppress the activity of other immune cells, thereby preventing excessive immune responses. Mutations or dysfunction in the FOXP3 gene can lead to severe autoimmune disorders such as IPEX syndrome, highlighting its importance in immune regulation. Additionally, FOXP3 has been implicated in various other biological processes, including cancer immunology, where its expression in tumor-infiltrating Tregs can influence the tumor microenvironment and affect the efficacy of immunotherapies.
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Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human tonsil. Anti-FOXP3 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung cancer. Anti-FOXP3 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse spleen. Anti-FOXP3 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat spleen. Anti-FOXP3 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
