FOXO4 Recombinant Rabbit mAb (S-3527-19)

Catalog Number: S0B6708 Application: WB, IHC-P, ChIP Reactivity: Hu Conjugation: Unconjugated Brand: Starter

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$100 USD

Size: 25μl 100μl

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Product Details

Product Specification

Host	Rabbit
Antigen	FOXO4
Immunogen	Synthetic Peptide
Location	Cytoplasm, Nucleus
Accession	P98177
Clone Number	S-3527-19
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P, ChIP
Reactivity	Hu
Positive Sample	Jurkat, U937, Ramos
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000-1:2000	Hu
IHC-P	1:2000	Hu
ChIP	1:20-1:50	Hu

Background

FOXO4 (Forkhead Box O4) is a transcription factor belonging to the FOXO family of proteins, which function as critical regulators of cellular stress responses, metabolism, longevity, and apoptosis. As a key downstream effector of the PI3K-AKT signaling pathway, FOXO4 activity is tightly controlled by post-translational modifications, particularly phosphorylation by AKT, which promotes its nuclear exclusion and cytoplasmic retention, thereby inhibiting its transcriptional functions. Upon activation by various stress stimuli—including oxidative stress, DNA damage, and nutrient deprivation—FOXO4 translocates to the nucleus where it binds to specific DNA sequences (FKHR recognition motifs) to modulate the expression of target genes involved in cell cycle arrest (p21, GADD45), DNA repair, autophagy, and pro-apoptotic pathways (BIM, FASL). Notably, FOXO4 has garnered significant attention in aging research due to its role in cellular senescence; the FOXO4-DRI (D-Retro-Inverso) peptide, which disrupts the interaction between FOXO4 and p53, has been demonstrated to selectively induce apoptosis in senescent cells, thereby extending healthspan and lifespan in aged mice. Additionally, FOXO4 participates in diverse physiological processes including glucose homeostasis, adipogenesis, and stem cell maintenance, while its dysregulation has been implicated in various pathologies such as cancer, where it can function as either a tumor suppressor or promoter depending on cellular context, as well as in neurodegenerative diseases and metabolic disorders.

Picture

Western Blot

WB result of FOXO4 Recombinant Rabbit mAb
Primary antibody: FOXO4 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Jurkat whole cell lysate 20 µg
Lane 2: U937 whole cell lysate 20 µg
Lane 3: Ramos whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 54 kDa
Observed MW: 58 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-FOXO4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human colon. Anti-FOXO4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human placenta. Anti-FOXO4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human tonsil. Anti-FOXO4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-FOXO4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human lung cancer. Anti-FOXO4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

ChIP

Chromatin immunoprecipitation (ChIP) was performed on Jurkat cross–linked with 1% formaldehyde for 10 min, then chromatin was fragmented by sonication. Parallel reactions used FOXO4 Recombinant Rabbit mAb (S-3527-19) and Rabbit mAb IgG Isotype Control (SDT-R173) at 1:20 for immunoprecipitation.
Post-immunoprecipitation, both samples were washed, eluted, and cross-links reversed. Purified DNA was analyzed by qPCR.
qPCR showed the enrichment of SOD2, MMP9 and SAT-α in FOXO4 Recombinant Rabbit mAb
(S-3527-19)-immunoprecipitated sample.