FoxO1 Recombinant Rabbit mAb (S-5118)

Catalog Number: S0B6970 Application: WB, IHC-P Reactivity: Hu, Ms, Rt Conjugation: Unconjugated Brand: Starter

Price:

$100 USD

Size: 25μl 100μl

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Product Details

Product Specification

Host	Rabbit
Antigen	FoxO1
Synonyms	Forkhead box protein O1; Forkhead box protein O1A; Forkhead in rhabdomyosarcoma; FKHR; FOXO1A; FOXO1
Location	Cytoplasm, Nucleus
Accession	Q12778
Clone Number	S-5118
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P
Reactivity	Hu, Ms, Rt
Positive Sample	293T, C6, COS-7
Predicted Reactivity	Mk
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000	Hu, Ms, Rt
IHC-P	1:500	Hu, Ms, Rt

Background

FoxO1 (Forkhead box protein O1) is a critical transcription factor belonging to the FoxO subfamily of the forkhead box family, playing a pivotal role in regulating cellular processes such as apoptosis, cell cycle progression, DNA damage repair, oxidative stress resistance, and metabolism, particularly in glucose homeostasis and lipid metabolism. Primarily regulated by the PI3K/Akt signaling pathway, FoxO1 undergoes phosphorylation in response to insulin or growth factors, leading to its exclusion from the nucleus and subsequent inhibition of its transcriptional activity; conversely, under conditions of low insulin or cellular stress, dephosphorylated FoxO1 translocates into the nucleus where it binds to specific DNA sequences to activate target genes like PEPCK and G6Pase, thereby promoting gluconeogenesis in the liver. Beyond its metabolic functions, FoxO1 is essential for maintaining stem cell quiescence, regulating immune responses, and suppressing tumorigenesis, making it a significant molecule of interest in understanding diseases such as type 2 diabetes, cancer, and aging-related disorders.

Picture

Western Blot

WB result of FoxO1 Recombinant Rabbit mAb
Primary antibody: FoxO1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HL-60 whole cell lysate 20 µg
Lane 2: 293T whole cell lysate 20 µg
Negative control: HL-60 whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 70 kDa
Observed MW: 68-80 kDa

WB result of FoxO1 Recombinant Rabbit mAb
Primary antibody: FoxO1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 70 kDa
Observed MW: 68-80 kDa

WB result of FoxO1 Recombinant Rabbit mAb
Primary antibody: FoxO1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 70 kDa
Observed MW: 68-80 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human tonsil. Anti-FoxO1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human cervix cancer. Anti-FoxO1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human colon cancer. Anti-FoxO1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse spleen. Anti-FoxO1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat spleen. Anti-FoxO1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.