FOXG1 Recombinant Rabbit mAb (S-5244)

Catalog Number: S0B60052 Application: WB, IHC-P Reactivity: Hu, Ms, Rt Conjugation: Unconjugated Brand: Starter

Price:

$100 USD

Size: 25μl 100µl

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Product Details

Product Specification

Host	Rabbit
Antigen	FOXG1
Synonyms	Forkhead box protein G1; Brain factor 1 (BF-1; BF1); Brain factor 2 (BF-2; BF2; hBF-2); Forkhead box protein G1A; Forkhead box protein G1B; Forkhead box protein G1C; FKH2; FKHL1; FKHL2; FKHL3; FKHL4; FOXG1A; FOXG1B; FOXG1C
Location	Nucleus
Accession	P55316
Clone Number	S-5244
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P
Reactivity	Hu, Ms, Rt
Positive Sample	NCI-H69, HEK-293, U-87 MG, mouse brain, C6, rat brain
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000	Hu, Ms, Rt
IHC-P	1:2000	Hu, Ms, Rt

Background

FOXG1 (Forkhead box G1) is a winged-helix transcription factor essential for vertebrate forebrain development, acting as a pioneer factor that opens chromatin and orchestrates the earliest steps of telencephalon specification by repressing dorsalizing genes, promoting FGF8 expression, and directing the temporal progression of cortical progenitor proliferation, neuronal layer fate transitions, oligodendrocyte maturation and postnatal myelination through dosage-sensitive interactions with morphogen signals, histone-modifying complexes and post-translational phosphorylation that control its nuclear–cytoplasmic shuttling, so that even modest alterations in FOXG1 levels produce a spectrum of neurodevelopmental disorders ranging from microcephaly and corpus callosum agenesis to severe encephalopathy with autism-like behaviors.

Picture

Western Blot

WB result of FOXG1 Recombinant Rabbit mAb
Primary antibody: FOXG1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NCI-H69 whole cell lysate 20 µg
Lane 2: HEK-293 whole cell lysate 20 µg
Lane 3: U-87 MG whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 52 kDa
Observed MW: 58 kDa

WB result of FOXG1 Recombinant Rabbit mAb
Primary antibody: FOXG1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse brain lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 52 kDa
Observed MW: 58 kDa
This blot was developed with high sensitivity substrate

WB result of FOXG1 Recombinant Rabbit mAb
Primary antibody: FOXG1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: rat brain lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 52 kDa
Observed MW: 58 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human glioma. Anti-FOXG1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-FOXG1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-FOXG1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat E14 embryo. Anti-FOXG1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.