FOLR1 Recombinant Rabbit mAb (SDT-3635-124)

Catalog Number: S0B2484 Application: WB, IHC-P Reactivity: Hu, Ms, Rt Conjugation: Unconjugated Brand: Starter

Price:

$100 USD

Size: 25μl 100µl 500µl

Quantity:

For shipping services or bulk orders, you may request a quotation.

Secure checkout with

View full details

Product Details

Product Specification

Host	Rabbit
Antigen	FOLR1
Synonyms	Folate receptor alpha; FR-alpha; Adult folate-binding protein (FBP); Folate receptor 1; Folate receptor; adult; KB cells FBP; Ovarian tumor-associated antigen MOv18; FOLR
Immunogen	Recombinant Protein
Location	Secreted, Endosome, Cell membrane
Accession	P15328
Clone Number	SDT-3635-124
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P
Reactivity	Hu, Ms, Rt
Positive Sample	JAR, HeLa, SK-OV-3, OVCAR-3
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000	Hu, Ms
IHC-P	1:500	Hu, Ms, Rt

Background

Human folate receptor alpha (FOLR1) is a glycoprotein that plays a crucial role in the transport of folate, an essential vitamin, into cells. This receptor is highly expressed in certain tissues and is particularly notable for its involvement in various physiological processes including embryonic development and cellular proliferation. Its overexpression has been observed in numerous cancers, such as ovarian, lung, and breast cancer, making it a potential target for cancer diagnosis and targeted therapy. By binding to folate or folate analogs, FOLR1 facilitates the internalization of these molecules via endocytosis, thereby providing cells with the necessary folate for metabolic functions like DNA synthesis and repair.

Picture

Western Blot

WB result of FOLR1 Recombinant Rabbit mAb
Primary antibody: FOLR1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: K562 whole cell lysate 20 µg
Lane 2: JAR whole cell lysate 20 µg
Lane 3: HeLa whole cell lysate 20 µg
Lane 4: SK-OV-3 whole cell lysate 20 µg
Lane 5: OVCAR-3 whole cell lysate 20 µg
Negative control: K562 whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 30 kDa
Observed MW: 36 kDa

WB result of FOLR1 Recombinant Rabbit mAb
Primary antibody: FOLR1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse kidney lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 30 kDa
Observed MW: 35 kDa

WB result of FOLR1 Recombinant Rabbit mAb
Primary antibody: FOLR1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat kidney lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 30 kDa
Observed MW: 32 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human kidney. Anti-FOLR1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human ovarian cancer (case 1). Anti-FOLR1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human ovarian cancer (case 2). Anti-FOLR1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse kidney. Anti-FOLR1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat kidney. Anti-FOLR1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.