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FLAG Tag Recombinant Rabbit mAb (APC Conjugate) (S-SC044)

FLAG Tag Recombinant Rabbit mAb (APC Conjugate) (S-SC044)

Catalog Number: S0B60687 Application: ICFCM Brand: Starter
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$100 USD
$100 USD
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Product Details

Product Specification


Host Rabbit
Antigen FLAG tag
Clone Number S-SC044
Antibody Type Recombinant mAb
Isotype IgG
Application ICFCM
Purification Protein A
Concentration 0.2 mg/ml
Conjugation APC
Physical Appearance Liquid
Storage Buffer

PBS, 1% BSA, 0.09% sodium azide
Stability & Storage

12 months from date of receipt / reconstitution, 2 to 8 °C as supplied

Dilution


application dilution species
FCM 1:20 Species Independent

Background

FLAG-tag, or FLAG octapeptide, or FLAG epitope, is a peptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence DYKDDDDK (where D=aspartic acid, Y=tyrosine, and K=lysine). It is one of the most specific tags and it is an artificial antigen to which specific, high affinity monoclonal antibodies have been developed and hence can be used for protein purification by affinity chromatography and also can be used for locating proteins within living cells. FLAG-tag has been used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. FLAG-tag can also be used in the isolation of protein complexes with multiple subunits.

Picture

FC

Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized Histone H3-FLAG Tag transfected 293F cells (Human embryonic kidney epithelial cell) / (right panel) compared with untransfected 293F cells / (left panel). Cells were labelled with anti-FLAG tag (APC Conjugate) Antibody at 1/20 (1 μg) dilution. Then cells were stained with anti-Histone H3 antibody at 1/2000 (0.1 μg) dilution (S0B1521). Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.