Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized Flag Tag transfected 293T cells (Human embryonic kidney epithelial cell) / (right panel) compared with untransfected 293T cells / (left panel). labelling SDT FLAG Tag Recombinant Rabbit mAb (Alexa Fluor® 647 Conjugate) (S-SC044) at 1/1000 dilution (0.1 μg). Then cells were stained with Histone H3(Alexa Fluor® 488 Conjugate) (S0B1521) at 1/2000 (0.1 μg) dilution. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
FLAG Tag Recombinant Rabbit mAb (Alexa Fluor® 647 Conjugate) (S-SC044)
FLAG Tag Recombinant Rabbit mAb (Alexa Fluor® 647 Conjugate) (S-SC044)
Price:
Regular price
$180 USD
Regular price
Sale price
$180 USD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | FLAG tag |
| Clone Number | S-SC044 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | ICC, ICFCM |
| Purification | Protein A |
| Concentration | 1 mg/ml |
| Conjugation | Alexa Fluor® 647 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| ICC | 1:500 | Species Independent |
| ICFCM | 1:1000 | Species Independent |
Background
FLAG-tag, or FLAG octapeptide, or FLAG epitope, is a peptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence DYKDDDDK (where D=aspartic acid, Y=tyrosine, and K=lysine). It is one of the most specific tags and it is an artificial antigen to which specific, high affinity monoclonal antibodies have been developed and hence can be used for protein purification by affinity chromatography and also can be used for locating proteins within living cells. FLAG-tag has been used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. FLAG-tag can also be used in the isolation of protein complexes with multiple subunits.
Picture
Picture
FC
Immunocytochemistry
ICC shows positive staining in FLAG Tag transfected 293F cells (top panel) and negative staining in vector-transfected 293F cells (below panel). Anti- FLAG Tag (Alexa Fluor® 647 Conjugate) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
