Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized Histone H3-FLAG Tag transfected 293F cells (Human embryonic kidney epithelial cell) / (right panel) compared with untransfected 293F cells / (left panel). Cells were labelled with anti-FLAG tag (PE Conjugate) Antibody at 1/200 (0.1 μg) dilution. Then cells were stained with anti-Histone H3 antibody at 1/2000 (0.1 μg) dilution (S0B1521). Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
FLAG Tag Recombinant Rabbit mAb (PE Conjugate) (S-SC044)
FLAG Tag Recombinant Rabbit mAb (PE Conjugate) (S-SC044)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Clone Number | S-SC044 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | FCM, ICFCM |
| Reactivity | Species Independent |
| Purification | Protein A |
| Concentration | 0.2 mg/ml |
| Conjugation | PE |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.09% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| ICFCM | 1:200 | Species Independent |
Background
FLAG-tag, or FLAG octapeptide, or FLAG epitope, is a peptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence DYKDDDDK (where D=aspartic acid, Y=tyrosine, and K=lysine). It is one of the most specific tags and it is an artificial antigen to which specific, high affinity monoclonal antibodies have been developed and hence can be used for protein purification by affinity chromatography and also can be used for locating proteins within living cells. FLAG-tag has been used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. FLAG-tag can also be used in the isolation of protein complexes with multiple subunits.
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