WB result of FITM1 Recombinant Rabbit mAb
Primary antibody: FITM1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: unboiled BXPC-3 whole cell lysate 20 µg
Lane 2: unboiled RD whole cell lysate 20 µg
Negative control: unboiled BXPC-3 whole cell lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 32 kDa
Observed MW: 24 kDa
Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | FITM1 |
| Synonyms | Fat storage-inducing transmembrane protein 1; FIT1 |
| Immunogen | Synthetic Peptide |
| Location | Endoplasmic reticulum |
| Accession | A5D6W6 |
| Clone Number | S-3819-65 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | RD, mouse heart, mouse skeletal muscle, rat heart |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
Background
FITM1 (Fat Storage-Inducing Transmembrane Protein 1) is an endoplasmic reticulum-resident protein that plays a key role in lipid metabolism by promoting the formation and growth of lipid droplets. It facilitates the partitioning of neutral lipids into storage organelles, thereby protecting cells from lipotoxicity. FITM1 is widely expressed in various tissues and is considered important for maintaining cellular energy homeostasis and metabolic health.
Picture
Picture
Western Blot
WB result of FITM1 Recombinant Rabbit mAb
Primary antibody: FITM1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: unboiled mouse heart lysate 20 µg
Lane 2: unboiled mouse skeletal muscle lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 32 kDa
Observed MW: 24 kDa
WB result of FITM1 Recombinant Rabbit mAb
Primary antibody: FITM1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: unboiled rat heart lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 32 kDa
Observed MW: 24 kDa
