Flow cytometric analysis of human peripheral blood cells labeling FITC Mouse IgG2a Isotype Control Antibody at 1/20 (1 μg) dilution (right Panel) compared with unlabeled control (cells without incubation with primary antibody and secondary antibody) (left Panel). Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
FITC Rat IgG2a Isotype Control (S-R483)
FITC Rat IgG2a Isotype Control (S-R483)
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Product Details
Product Details
Product Specification
| Host | Rat |
| Clone Number | S-R483 |
| Antibody Type | Rat mAb |
| Isotype | IgG2a |
| Application | FCM, ICFCM |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | FITC |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.09% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Background
Isotype control antibodies, to estimate the nonspecific binding of target. Use at concentrations comparable to those of the specific antibody of interest.
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FC
Flow cytometric analysis of A431 (Human epidermoid carcinoma epithelial cell) labeling FITC Mouse IgG2a Isotype Control Antibody at 1/20 (1 μg) dilution (right Panel) compared with unlabeled control (cells without incubation with primary antibody and secondary antibody) (left Panel). Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
Flow cytometric analysis of 4% paraformaldehyde fixed 0.1% Tween permeabilized human PBMCs (human peripheral blood mononuclear cells) labelling FITC Mouse IgG2a Isotype Antibody at 1/20 (1 μg) dilution (right Panel) compared with unlabeled control (cells without incubation with primary antibody and secondary antibody) (left Panel). Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
Flow cytometric analysis of 4% paraformaldehyde fixed 0.1% Tween permeabilized 293F (Human embryonic kidney epithelial cell) labelling FITC Mouse IgG2a Isotype control Antibody at 1/20 (1 μg) dilution (right Panel) compared with unlabeled control (cells without incubation with primary antibody and secondary antibody) (left Panel). Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
