Flow cytometric analysis of Mouse TER-119/Erythroid expression on C57BL/6 mouse peritoneal exudate cells. C57BL/6 mouse peritoneal exudate cells were stained with SDT FITC Rat Anti-Mouse TER-119/Erythroid Antibody (Red line histogram) at 1 μg/test, cells without incubation with primary antibody and secondary antibody (Blue line histogram) was used as unlabelled control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
FITC Rat Anti-Mouse TER-119/Erythroid Cells Antibody (TER-119)
FITC Rat Anti-Mouse TER-119/Erythroid Cells Antibody (TER-119)
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Product Details
Product Details
Product Specification
| Host | Rat |
| Synonyms | Lymphocyte antigen 76; Ly76; Ly-76; Ter119 |
| Location | Cell membrane |
| Clone Number | TER-119 |
| Antibody Type | Rat mAb |
| Isotype | IgG2b,k |
| Application | FCM |
| Reactivity | Ms |
| Positive Sample | C57BL/6 mouse peritoneal exudate cells |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | FITC |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| FCM | 5 μl per million cells in 100μl volume | Ms |
Background
The TER-119 protein is one of the most specific and important cell surface markers for the murine erythroid lineage. It specifically recognizes an antigen associated with glycophorin A on the red blood cell membrane. Throughout erythroid development, from early basophilic erythroblasts to late-stage reticulocytes, it is consistently highly expressed, making it a critical molecular marker for identifying, sorting, and studying murine erythroid cells (such as erythrocytes, progenitor cells, and precursors). This protein belongs to the Ly-6 antigen superfamily, and its antibody (e.g., the TER-119 monoclonal antibody) is an indispensable tool in experimental hematology for the isolation and identification of red blood cells via flow cytometry or immunomagnetic beads, as well as for research into blood diseases like anemia and leukemia.
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