Flow cytometric analysis of Mouse Siglec-E expression on BALB/c mouse splenocytes. BALB/c mouse splenocytes were stained with Pacific Blue™ Rat Anti-Mouse Ly-6G/Ly-6C antibody and either FITC Rat IgG2b Isotype Control (Left panel) or SDT FITC Rat Anti-Mouse Siglec-E Antibody (Right panel) at 5 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
FITC Rat Anti-Mouse Siglec-E Antibody (S-3881)
FITC Rat Anti-Mouse Siglec-E Antibody (S-3881)
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | Siglec-E |
| Synonyms | Sialic acid binding Ig-like lectin E; Siglece; Siglecl1 |
| Location | Membrane |
| Accession | Q91Y57 |
| Clone Number | S-3881 |
| Antibody Type | Rat mAb |
| Isotype | IgG2b |
| Application | FCM |
| Reactivity | Ms |
| Positive Sample | BALB/c mouse splenocytes |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | FITC |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Ms |
Background
Siglec-E, the murine orthologue of human Siglec-9, is a sialic acid-binding immunoglobulin-like lectin expressed predominantly on myeloid cells (macrophages, neutrophils, dendritic cells) that functions as a critical immunosuppressive checkpoint by recruiting SHP-1/2 phosphatases upon engagement of sialoglycan ligands, thereby attenuating TLR4- and TRIF-dependent signaling, dampening NF-κB activation and pro-inflammatory cytokine production, and exerting neuroprotective and anti-atherogenic effects through inhibition of microglial activation and CD36-mediated oxidized LDL uptake.
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