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FITC Rat Anti-Mouse IL-7Rα (CD127) Antibody (S-3263)

FITC Rat Anti-Mouse IL-7Rα (CD127) Antibody (S-3263)

Catalog Number: S0B8267 Application: FCM Reactivity: Mouse Conjugation: FITC Brand: Starter
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Product Details

Product Specification


Host Rat
Antigen IL-7Rα (CD127)
Synonyms Interleukin-7 receptor subunit alpha; IL-7 receptor subunit alpha; IL-7R subunit alpha; IL-7R-alpha; IL-7RA; Il7r
Location Membrane
Accession P16872
Clone Number S-3263
Antibody Type Rat mAb
Isotype IgG2a,k
Application FCM
Reactivity Ms
Positive Sample BALB/c mouse splenocytes
Purification Protein G
Concentration 0.2 mg/ml
Conjugation FITC
Physical Appearance Liquid
Storage Buffer

PBS, 1% BSA, 0.3% Proclin 300
Stability & Storage

12 months from date of receipt / reconstitution, 2 to 8 °C as supplied

Dilution


application dilution species
FCM 5μl per million cells in 100μl volume Ms

Background

Interleukin-7 receptor α chain (IL-7Rα, CD127) is a 75 kDa type-I transmembrane glycoprotein that forms the cytokine-binding subunit of both the IL-7 receptor (IL-7R) and the thymic stromal lymphopoietin receptor (TSLPR); upon binding IL-7 it heterodimerizes with the common gamma chain (γc, CD132) to initiate JAK1/JAK3-mediated phosphorylation of STAT5, thereby driving survival, proliferation and differentiation of T, B and innate lymphoid cells, while TSLP engagement pairs IL-7Rα with the TSLPR chain to modulate type-2 immunity.

Picture

FC

Flow cytometric analysis of Mouse IL-7Rα (CD127) expression on BALB/c mouse splenocytes. BALB/c mouse splenocytes were stained with Brilliant Violet 421™ Rat Anti-Mouse CD3 antibody and either FITC Rat IgG2a, κ Isotype Control (Left panel) or SDT FITC Rat Anti-Mouse IL-7Rα (CD127) Antibody (Right panel) at 5 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.