Flow cytometric analysis of Mouse CD23 expression on BALB/c mouse splenocytes. BALB/c mouse splenocytes were stained with Brilliant Violet 421™ Rat Anti-Mouse CD19 Antibody and either FITC Rat IgG2a, κ Isotype Control (Left panel) or SDT FITC Rat Anti-Mouse CD23 Antibody (Right panel) at 5μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
FITC Rat Anti-Mouse CD23 Antibody (S-R672)
FITC Rat Anti-Mouse CD23 Antibody (S-R672)
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | CD23 |
| Synonyms | Low affinity immunoglobulin epsilon Fc receptor; Fc-epsilon-RII; Lymphocyte IgE receptor; Fcer2a; Fcer2 |
| Location | Secreted, Cell membrane |
| Accession | P20693 |
| Clone Number | S-R672 |
| Antibody Type | Rat mAb |
| Isotype | IgG2a,k |
| Application | FCM |
| Reactivity | Ms |
| Positive Sample | BALB/c mouse splenocytes |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | FITC |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Ms |
Background
CD23, also known as the low-affinity IgE receptor (FcεRII), is a 45 kDa type II integral membrane glycoprotein that plays a crucial role in regulating IgE production and B-cell differentiation. It is expressed on various immune cells, including mature B cells, monocytes, eosinophils, follicular dendritic cells, and platelets. CD23 exists in two isoforms, CD23a and CD23b, which differ in their amino acid sequences and functions. The molecule can also be cleaved into a soluble form (sCD23) that retains IgE-binding capabilities.
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