Flow cytometric analysis of IL-10 expression in human PBMC polarized with Th2 Polarization Kit, Human (UA090018). Th2 polarized human PBMC were harvested and restimulated for 5 hr with PMA and Ionomycin in the presence of Brefeldin A (right Panel) or unstimulated (left Panel).
The cells were harvested and fixed with 4% PFA and permeabilized with Intracellular Fixation & Permeabilization Buffer Set. The cells were then stained with Phycoerythrin Mouse Anti-Human CD4 Antibody and SDT FITC Rat Anti-Human IL-10 Antibody at 5μl/test. Total viable cells, as determined by Fixable Viability Dye 452 (S0D0021), were used for analysis. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
FITC Rat Anti-Human IL-10 Antibody (S-3081)
FITC Rat Anti-Human IL-10 Antibody (S-3081)
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | IL-10 |
| Synonyms | Interleukin-10; Cytokine synthesis inhibitory factor (CSIF); IL10 |
| Location | Secreted |
| Accession | P22301 |
| Clone Number | S-3081 |
| Antibody Type | Rat mAb |
| Isotype | IgG2a,k |
| Application | ICFCM |
| Reactivity | Hu |
| Positive Sample | Th2 polarized human PBMC |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | FITC |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| ICFCM | 5μl per million cells in 100μl volume | Hu |
Background
Interleukin-10 (IL-10) is a cytokine that plays a crucial role in regulating immune responses. It is primarily produced by various immune cells, including monocytes, macrophages, and regulatory T cells. IL-10 has potent anti-inflammatory properties, as it inhibits the production of pro-inflammatory cytokines such as IL-1, TNF-α, and IFN-γ from activated macrophages and T helper 1 cells. By doing so, it helps to prevent excessive inflammation and tissue damage. Additionally, IL-10 promotes the differentiation of regulatory T cells and enhances the function of B cells, contributing to the maintenance of immune homeostasis. Dysregulation of IL-10 production has been implicated in various diseases, including autoimmune disorders and certain types of cancer, highlighting its importance in both health and disease.
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