Flow cytometric analysis of Human CD14 expression on human peripheral blood. Human peripheral blood was stained with FITC Rabbit IgG Isotype Control (Left panel) or SDT FITC Rabbit Anti-Human CD14 Antibody (Right panel) at 5μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
FITC Rabbit Anti-Human CD14 Antibody (S-395-8)
FITC Rabbit Anti-Human CD14 Antibody (S-395-8)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | CD14 |
| Synonyms | Myeloid cell-specific leucine-rich glycoprotein; Monocyte differentiation antigen CD14; FMC32lipopolysaccharide (LPS) |
| Immunogen | Synthetic Peptide |
| Location | Secreted, Golgi Apparatus, Cell membrane |
| Accession | P08571 |
| Clone Number | S-395-8 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | human peripheral blood |
| Purification | Protein A |
| Concentration | 0.05mg/ml |
| Conjugation | FITC |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Hu |
Background
CD14 (cluster of differentiation 14) is a human protein made mostly by macrophages as part of the innate immune system. It helps to detect bacteria in the body by binding lipopolysaccharide (LPS), a pathogen-associated molecular pattern (PAMP). CD14 exists in two forms, one anchored to the membrane by a glycosylphosphatidylinositol (GPI) tail (mCD14), the other a soluble form (sCD14). Soluble CD14 either appears after shedding of mCD14 (48 kDa) or is directly secreted from intracellular vesicles (56 kDa).
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