Flow cytometric analysis of Rat CD90 expression on Lewis Rat splenocytes. Lewis Rat splenocytes were stained with Brilliant Violet 605™ Mouse Anti-Rat CD3 Antibody and either FITC Mouse IgG1, κ Isotype Control (Left panel) or SDT FITC Mouse Anti-Rat CD90 Antibody (Right panel) at 5μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
FITC Mouse Anti-Rat CD90 Antibody (S-R687)
FITC Mouse Anti-Rat CD90 Antibody (S-R687)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CD90 |
| Synonyms | Thy-1 membrane glycoprotein; Thy-1 antigen; Thy-1; Thy1 |
| Location | Cell membrane |
| Accession | P01830 |
| Clone Number | S-R687 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1,k |
| Application | FCM |
| Reactivity | Rt |
| Positive Sample | Lewis Rat splenocytes |
| Purification | Protein G |
| Concentration | 0.05mg/ml |
| Conjugation | FITC |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Rt |
Background
CD90, also known as Thy-1, is a glycosylphosphatidylinositol (GPI)-anchored glycoprotein and a member of the immunoglobulin superfamily with a molecular weight of 25–35 kDa. It is primarily expressed in leukocytes, fibroblasts, and various stromal cells. CD90 plays a crucial role in cell–cell and cell–matrix interactions, and it is involved in processes such as integrin-mediated signaling, cell adhesion, and cellular extravasation. In the context of hematopoietic stem cells, CD90 is often used as a marker for identifying primitive hematopoietic progenitor cells, particularly in combination with other markers like CD34. Additionally, CD90 is expressed on human mesenchymal stem cells (MSCs) from bone marrow and other sources, highlighting its significance in stem cell biology.
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