Lewis Rat peritoneal exudates cells were fixed with 4% PFA and permeabilization with the 0.1% Tween. Cells were then intracellularly stained with Phycoerythrin Mouse Anti-Rat CD11b and SDT FITC Mouse Anti-Rat CD68 Antibody Antibody at 5 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
FITC Mouse Anti-Rat CD68 Antibody (S-R674)
FITC Mouse Anti-Rat CD68 Antibody (S-R674)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CD68 |
| Location | Lysosome, Endosome, Cell membrane |
| Accession | Q4FZY1 |
| Clone Number | S-R674 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1,k |
| Application | ICFCM |
| Reactivity | Rt |
| Positive Sample | Lewis Rat peritoneal exudates cells |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | FITC |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| ICFCM | 5μl per million cells in 100μl volume | Rt |
Background
The CD68 protein is a heavily glycosylated transmembrane protein belonging to the lysosome-associated membrane glycoprotein (LAMP) family, primarily serving as a macrophage marker. With a molecular weight of approximately 110 kDa, it contains scavenger receptor-like cysteine-rich domains and participates in innate immune responses by binding specific ligands such as oxidized low-density lipoprotein (oxLDL). Localized to lysosomal and endosomal membranes, CD68 plays a crucial role in antigen presentation, inflammatory responses, and tissue repair. It is commonly detected via immunohistochemistry (e.g., using the PG-M1 antibody) and is widely utilized to identify tumor-associated macrophages (TAMs) and assess pathological progression in chronic inflammatory diseases, including atherosclerosis and rheumatoid arthritis.
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