Flow cytometric analysis of Human CD73 expression on human PBMC (human peripheral blood mononuclear cells). Human PBMC were stained with Brilliant Violet 421™ Mouse Anti-Human CD3 Antibody and either FITC Mouse IgG2b, κ Isotype Control (Left panel) or SDT FITC Mouse Anti-Human CD73 Antibody (Right panel) at 5μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
FITC Mouse Anti-Human CD73 Antibody (S-715-115)
FITC Mouse Anti-Human CD73 Antibody (S-715-115)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CD73 |
| Synonyms | 5'-nucleotidase; 5'-NT; 5'-deoxynucleotidase; Ecto-5'-nucleotidase; IMP-specific 5'-nucleotidase; Thymidylate 5'-phosphatase; NT5E; NT5; NTE |
| Immunogen | Recombinant Protein |
| Location | Cell membrane |
| Accession | P21589 |
| Clone Number | S-715-115 |
| Antibody Type | Mouse mAb |
| Isotype | IgG2b,k |
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | human PBMC |
| Purification | Protein A |
| Concentration | 0.05mg/ml |
| Conjugation | FITC |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Hu |
Background
NT5E (CD73) is a surface enzyme which is expressed on multiple cells. This enzyme mediates the gradual hydrolysis of the autocrine and paracrine danger signals of ATP and ADP to anti-inflammatory adenosine. Immune suppression mediated by adenosinergic pathways is very important for maintaining immune system homeostasis. Immune suppressive functions of T regulatory cells are also dependent on CD73 expression. CD73 also occurs on anergic CD4 + T cells, thereby maintaining self-tolerance to healthy tissues as well as protecting the fetus from the mother's immune system during pregnancy.
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