Flow cytometric analysis of Human CD31 expression on Human peripheral blood. Human peripheral blood was stained with Brilliant Violet 421™ Mouse Anti-Human CD14 Antibody and either FITC Mouse IgG1, κ Isotype Control (left Panel) and SDT FITC Mouse Anti-Human CD31 Antibody (right Panel) at 5 μl/test. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
FITC Mouse Anti-Human CD31 Antibody (WM59)
FITC Mouse Anti-Human CD31 Antibody (WM59)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CD31 |
| Synonyms | Platelet endothelial cell adhesion molecule; PECAM-1; EndoCAM; GPIIA'; PECA1; PECAM1 |
| Location | Cell membrane |
| Accession | P16284 |
| Clone Number | WM59 |
| Antibody Type | Mouse mAb |
| Isotype Control | S0B1529 |
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | Human peripheral blood cells |
| Purification | Protein A |
| Concentration | 0.2 mg/ml |
| Conjugation | FITC |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.09% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Hu |
Background
CD31, also known as platelet endothelial cell adhesion molecule-1 (PECAM-1), is a 130 kDa transmembrane glycoprotein of the immunoglobulin superfamily composed of six extracellular Ig-like domains, a single transmembrane region, and a cytoplasmic tail with multiple phosphorylation sites, and it is constitutively expressed on endothelial cells, platelets, leukocytes, and hematopoietic stem cells, where it mediates homophilic and heterophilic cell–cell adhesion, facilitates leukocyte transendothelial migration, regulates vascular permeability, and plays an essential role in developmental and pathological angiogenesis by promoting endothelial tube formation and neovessel stabilization.
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