Flow cytometric analysis of Human CD28 expression on human PBMC (human peripheral blood mononuclear cell). Human PBMC were stained with Brilliant Violet 421™ Mouse Anti-Human CD3 antibody and either FITC Mouse IgG1 Isotype Control (Left panel) or SDT FITC Mouse Anti-Human CD28 Antibody (Right panel) at 1 μg/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
FITC Mouse Anti-Human/ Monkey CD28 Antibody (CD28.2)
FITC Mouse Anti-Human/ Monkey CD28 Antibody (CD28.2)
Price:
Regular price
$200 USD
Regular price
Sale price
$200 USD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CD28 |
| Synonyms | T-cell-specific surface glycoprotein CD28; TP44 |
| Location | Cell membrane |
| Accession | P10747 |
| Clone Number | CD28.2 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1 |
| Isotype Control | S0B1529 |
| Application | FCM |
| Reactivity | Hu, RhMk, CyMk |
| Positive Sample | Human PBMC |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | FITC |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2-8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Hu, RhMk, CyMk |
Background
CD28 (Cluster of Differentiation 28) is one of the proteins expressed on T cells that provide co-stimulatory signals required for T cell activation and survival. T cell stimulation through CD28 in addition to the T-cell receptor (TCR) can provide a potent signal for the production of various interleukins (IL-6 in particular). CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2) proteins. When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen-presenting cells (APCs). The CD86 expression on antigen-presenting cells is constitutive (expression is independent of environmental factors). It is generally reported, that CD28 is expressed on 50% of CD8+ T cells and more than 80% CD4+ T cells in human, but during the course of activation some T cells lose this molecule. In general, CD28 is a primary costimulatory molecule for T cell activation.
Picture
Picture
FC
Flow cytometric analysis of CD28 expression on Rhesus monkey peripheral blood cells. Rhesus macaque peripheral blood cells were stained with FITC Mouse IgG1, κ Isotype Control (left panel) or SDT FITC Mouse Anti- Human CD28 Antibody (right panel) at 1 μg/test. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
Flow cytometric analysis of CD28 expression on Cynomolgus monkey peripheral blood cells. Cynomolgus macaque peripheral blood cells were stained with FITC Mouse IgG1, κ Isotype Control (left panel) or SDT FITC Mouse Anti- Human CD28 Antibody (right panel) at 1 μg/test. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
