Flow cytometric analysis of human PBMC were stained with stimulated 2 days with 10 μg/ml PHA (Right panel) or unstimulated (Left panel) was stained with Brilliant Violet 421™ Mouse Anti-Human CD3 and SDT FITC Mouse Anti-Human CD274 Antibody Antibody at 5 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
FITC Mouse Anti-Human CD274 Antibody (MIH1)
FITC Mouse Anti-Human CD274 Antibody (MIH1)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CD274 |
| Location | Nucleus, Cell membrane |
| Accession | Q9NZQ7 |
| Clone Number | MIH1 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1,k |
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | PHA stimulated human PBMC |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | FITC |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Hu |
Background
CD274, also known as PD-L1 (Programmed Death-Ligand 1), is a ~33 kDa type I transmembrane glycoprotein of the immunoglobulin superfamily that functions as the key ligand for the inhibitory receptor PD-1 on T cells; upon IFN-γ–driven up-regulation on dendritic cells, macrophages, normal epithelia and many tumors, its engagement of PD-1 delivers a “stop” signal that blocks T-cell receptor signaling, suppressing proliferation, cytokine production and cytotoxic activity, thereby maintaining peripheral tolerance and preventing autoimmunity, but is exploited by cancers to evade immune destruction, making the PD-1/PD-L1 axis a central target for modern checkpoint-inhibitor immunotherapies.
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