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FITC Mouse Anti-Human CD235a Antibody (NNA7)

FITC Mouse Anti-Human CD235a Antibody (NNA7)

Catalog Number: S0B5379 Application: FCM Reactivity: Hu Conjugation: FITC Brand: Starter
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Product Details

Product Specification


Host Mouse
Antigen CD235a
Location Cell membrane
Accession P02724
Clone Number NNA7
Antibody Type Mouse mAb
Isotype IgG2a,k
Isotype Control FITC Mouse IgG2a, κ Isotype Control Antibody (S-844-17)
Application FCM
Reactivity Hu
Purification Protein A
Concentration 0.2 mg/ml
Conjugation FITC
Physical Appearance Liquid
Storage Buffer

PBS, 1% BSA, 0.3% Proclin 300
Stability & Storage 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.

Dilution


application dilution species
FCM 1.25μl per million cells in 100μl volume Hu

Background

CD235a protein, also known as Glycophorin A, is a major intrinsic membrane protein of human red blood cells and erythroid precursor cells. It is a type I sialoglycoprotein with a molecular weight of around 10 kD, present in the cell membrane as a homodimer. This protein serves as a carrier for the MN blood group antigens. It also plays a crucial role in maintaining the integrity and shape of the erythrocyte membrane by being a part of the ankyrin-1 complex. Additionally, CD235a acts as a receptor for several pathogens, including the influenza virus, Plasmodium falciparum erythrocyte-binding antigen 175 (EBA-175), and hepatitis A virus.

Picture

FC

Flow cytometric analysis of Human CD235a expression on Human Peripheral Blood cells. Human Peripheral Blood cells were stained with Brilliant Violet 786™ Mouse Anti-Human CD45 Antibody and either FITC Mouse IgG2a, κ Isotype Control (Left panel) or FITC Mouse Anti-Human CD235a Antibody (Right panel) at 1.25 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.