Flow cytometric analysis of Human CD15 expression on human peripheral blood leukocytes. Human peripheral blood leukocytes were stained with Brilliant Violet 421™ Rat Anti-Human/Mouse CD11b antibody and either FITC Mouse IgM Isotype Control (left panel) or SDT FITC Mouse Anti-Human CD15 Antibody (right panel) at 1.25μl/test. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
FITC Mouse Anti-Human CD15 Antibody (S-R463)
FITC Mouse Anti-Human CD15 Antibody (S-R463)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CD15 |
| Location | Cell membrane |
| Clone Number | S-R463 |
| Antibody Type | Mouse mAb |
| Isotype | IgM |
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | Human peripheral blood leukocytes |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | FITC |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 1.25μl per million cells in 100μl volume | Hu |
Background
CD15, also known as Lewis X or SSEA-1, is a carbohydrate epitope (Galβ1→4[Fucα1→3]GlcNAc) carried primarily on glycoproteins and glycolipids of myeloid cells, granulocytes, and certain lymphocyte subsets; it serves as a key adhesion molecule that mediates selectin-dependent leukocyte rolling during inflammation, acts as a marker of mature neutrophils and Hodgkin/Reed-Sternberg cells, and its differential expression is exploited in immunohistochemistry to distinguish myeloid leukemias from lymphoid malignancies and to identify stem-cell populations in normal and neoplastic tissues.
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