Flow Cytometric Analysis of Annexin V FITC staining. Jurkat cells (Human T cell leukemia T lymphocyte) were untreated (left panels) or treated (right panels) for 4 hours with 6 µM campotothecin. Cells were incubated with 1ug Annexin V FITC in a buffer containing propidium iodide (PI). Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
FITC-Labeled Annexin V His Tag Protein, Human
FITC-Labeled Annexin V His Tag Protein, Human
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Product Details
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Product Specification
| Species | Human |
| Synonyms | Annexin A5, Anchorin CII, Annexin V, Annexin-5, Calphobindin I (CPB-I), Endonexin II, Lipocortin V, Placental anticoagulant protein 4 (PP4), Placental anticoagulant protein I (PAP-I), Thromboplastin inhibitor, Vascular anticoagulant-alpha (VAC-alpha) |
| Accession | P08758 |
| Amino Acid Sequence | Met1-Asp320 with His Tag at C-Terminus |
| Expression System | E.coli |
| Molecular Weight | 33 kDa (Reducing) |
| Conjugation | FITC |
| Tag | His Tag |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, pH7.4 |
| Stability & Storage | · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. |
| Reference | 1. Nat Rev Mol Cell Biol. 2004 Nov;5(11):897-907. |
Background
Annexin V is a calcium-dependent phospholipid-binding protein that has been widely used as a molecular probe for detecting and quantifying apoptotic cells. Annexin V has a high affinity for phosphatidylserine, a phospholipid that is normally located on the inner leaflet of the plasma membrane but becomes exposed on the outer surface of the membrane during the early stages of apoptosis. By binding to phosphatidylserine, Annexin V can be conjugated with fluorophores or other detection molecules to label apoptotic cells for flow cytometry, fluorescence microscopy, or other imaging techniques. The use of Annexin V in apoptosis assays has become a standard method for studying cell death processes in research and clinical settings.
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