WB result of FIS1 Rabbit pAb
Primary antibody: FIS1 Rabbit pAb at 1/1000 dilution
Lane 1: HEK-293 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: Jurkat whole cell lysate 20 µg
Lane 4: HepG2 whole cell lysate 20 µg
Lane 5: A431 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 17 kDa
Observed MW: 17 kDa
FIS1 Rabbit Polyclonal Antibody
FIS1 Rabbit Polyclonal Antibody
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | FIS1 |
| Synonyms | Mitochondrial fission 1 protein; FIS1 homolog (hFis1); Tetratricopeptide repeat protein 11 (TPR repeat protein 11); TTC11 |
| Immunogen | Recombinant Protein |
| Location | Mitochondrion |
| Accession | Q9Y3D6 |
| Antibody Type | Polyclonal antibody |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Ms, Rt, Mk |
| Positive Sample | HEK-293, HeLa, Jurkat, HepG2, A431, EL4, PC-12, rat spleen, COS-7 |
| Purification | Immunogen Affinity |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:2000 | Hu, Ms, Rt, Mk |
Background
FIS1 (Mitochondrial Fission Protein 1) is a 17-kDa outer mitochondrial membrane protein that plays a central role in mitochondrial quality control by promoting organellar fission: under stress it recruits the GTPase DRP1 to form a constriction ring that severs mitochondria, while also inhibiting fusion proteins (MFN/OPA1), engaging mitophagy adaptors (TBC1D15/17) to clear damaged fragments, and interacting with BAP31 to amplify apoptotic signals, thereby governing mitochondrial morphology, energy metabolism, cell survival and—when dysregulated—tumorigenesis or neurodegeneration.
Picture
Picture
Western Blot
WB result of FIS1 Rabbit pAb
Primary antibody: FIS1 Rabbit pAb at 1/1000 dilution
Lane 1: EL4 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 17 kDa
Observed MW: 17 kDa
WB result of FIS1 Rabbit pAb
Primary antibody: FIS1 Rabbit pAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: rat spleen lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 17 kDa
Observed MW: 17 kDa
WB result of FIS1 Rabbit pAb
Primary antibody: FIS1 Rabbit pAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 17 kDa
Observed MW: 17 kDa
