WB result of Firefly Luciferase Rabbit pAb
Primary antibody: Firefly Luciferase Rabbit pAb at 1/1000 dilution
Lane 1: untreated 293T whole cell lysate 5 µg
Lane 2: 293T transfected with myc-His-Firefly Luciferase whole cell lysate 5 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 61 kDa
Observed MW: 60 kDa
Firefly Luciferase Rabbit Polyclonal Antibody
Firefly Luciferase Rabbit Polyclonal Antibody
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Firefly Luciferase |
| Synonyms | Luciferin 4-monooxygenase |
| Immunogen | Synthetic Peptide |
| Accession | P08659 |
| Isotype | IgG |
| Application | WB, IP |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | |
| IP | 1:50 |
Background
Firefly Luciferase, also known as Photinus pyralis luciferase (FLuc), is a widely used enzyme in bioluminescence imaging due to its well-characterized properties and high sensitivity in detecting light output. The enzyme catalyzes the oxidation of luciferin in the presence of oxygen, ATP, and Mg2+ to produce a yellow-green light with a peak emission at 560-578 nm. This bioluminescent reaction is highly efficient and does not require any additional substrates or cofactors, making it an ideal reporter for gene expression studies and imaging in living organisms.
Picture
Picture
Western Blot
IP
Firefly Luciferase Rabbit pAb at 1/50 dilution (1 µg) immunoprecipitating Firefly Luciferase in 0.4 mg 293T transfected with myc-His-Firefly Luciferase whole cell lysate.
Western blot was performed on the immunoprecipitate using Firefly Luciferase Rabbit pAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: 293T transfected with myc-His-Firefly Luciferase whole cell lysate 5 µg (Input)
Lane 2: Firefly Luciferase Rabbit pAb IP in 293T transfected with myc-His-Firefly Luciferase whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in 293T transfected with myc-His-Firefly Luciferase whole cell lysate
Predicted MW: 61 kDa
Observed MW: 60 kDa
