WB result of Fibronectin Recombinant Rabbit mAb
Primary antibody: Fibronectin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HepG2 whole cell lysate 20 µg
Lane 2: human serum lysate 5 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 262 kDa
Observed MW: 280 kDa
Fibronectin Recombinant Rabbit mAb (S-1475-79)
Fibronectin Recombinant Rabbit mAb (S-1475-79)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Fibronectin |
| Synonyms | FN, Cold-insoluble globulin (CIG), FN1 |
| Immunogen | Synthetic Peptide |
| Location | Secreted |
| Accession | P02751 |
| Clone Number | S-1475-79 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms |
| Predicted Reactivity | Bv, Hr, Dg, Rt |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | |
| IHC-P | 1:250 |
Background
Fibronectin is a glycoprotein that is found in the extracellular matrix of a variety of animal tissues. It plays a crucial role in cell adhesion, growth, migration, and differentiation. Fibronectin also helps to organize the extracellular matrix and is involved in various physiological and pathological processes, such as wound healing, embryogenesis, and cancer metastasis. It is produced by various cell types, including fibroblasts, endothelial cells, and macrophages, and is essential for the proper functioning of tissues and organs. Deficiencies or abnormalities in fibronectin can lead to various diseases and disorders.
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Western Blot
WB result of Fibronectin Recombinant Rabbit mAb
Primary antibody: Fibronectin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse plasma lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 262 kDa
Observed MW: 280 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human spleen. Anti-Fibronectin antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver. Anti-Fibronectin antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-Fibronectin antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung cancer. Anti-Fibronectin antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-Fibronectin antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human pancreatic cancer. Anti-Fibronectin antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
