The FGFR2 K659N activity was detected using HTRF technology. The reaction was performed by incubating the FGFR2 K659N protein, ATP and substrate at 25℃ for 60 min, adding the beads at 25℃ for 60 min, then reading ratio 665/620 with BMG.
Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | FGFR2[K659N] |
| Accession | P21802-1 |
| Amino Acid Sequence |
R399-T821(end) RMKNTTKKPDFSSQPAVHKLTKRIPLRRQVTVSAESSSSMNSNTPLVRITTRLSSTADTPMLAGVSEYELPEDPKWEFPRDKLTLGKPLGEGCFGQVVMAEAVGIDKDKPKEAVTVAVKMLKDDATEKDLSDLVSEMEMMKMIGKHKNIINLLGACTQDGPLYVIVEYASKGNLREYLRARRPPGMEYSYDINRVPEEQMTFKDLVSCTYQLARGMEYLASQKCIHRDLAARNVLVTENNVMKIADFGLARDINNIDYYKNTTNGRLPVKWMAPEALFDRVYTHQSDVWSFGVLMWEIFTLGGSPYPGIPVEELFKLLKEGHRMDKPANCTNELYMMMRDCWHAVPSQRPTFKQLVEDLDRILTLTTNEEYLDLSQPLEQYSPSYPDTRSSCSSGDDSVFSPDPMPYEPCLPQYPHINGSVKT |
| Expression System | Baculovirus-InsectCells |
| Molecular Weight | 74.6 kDa |
| Purity | >70% by SDS-PAGE |
| Endotoxin | <1EU/μg |
| Conjugation | Unconjugated |
| Tag | GST Tag |
| Physical Appearance | Liquid |
| Storage Buffer | 50 mM Tris, 150 mM NaCl, 1mM DTT, 20 mM reduced glutathione, 10% glycerol, 0.05% Brij35, pH 7.5 |
| Stability & Storage | Stable for12 months upon stored at -80℃ from the date of receipt. And avoid repeatedfreeze-thaws cycles. |
Picture
Picture
Bioactivity


SDS-PAGE

![FGFR2[K659N] Protein](http://www.antbioinc.com/cdn/shop/files/f0ce82bd-59d4-4d4e-ad28-0c9ba2ebd71f.png?v=1787663048&width=1445)