Measured in a cell proliferation assay using Balb/c 3T3 mouse fibroblast cells, the EC50 for this effect is 0.0091-0.019ng/ml.
*Recombinant FGF-basic Heat Stable protein was incubated at 4°C or 37°C for 3 days in media. The protein retained similar activity indicating that the Heat Stable protein
has increased thermal stability.
FGF-basic(154aa) Heat Stable Protein, Human
FGF-basic(154aa) Heat Stable Protein, Human
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Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | bFGF, FGF-2, Heparin-binding growth factor 2, Basic fibroblast growth factor, FGFB, HBGF-2, FGF2, Fibroblast Growth Factor 2 (Basic) |
| Accession | P09038 |
| Amino Acid Sequence | Ala135-Ser288 with proprietary amino acid modifications |
| Expression System | E.coli |
| Molecular Weight | 14-15 kDa (Reducing) |
| Purity | >95% by SDS-PAGE & HPLC. |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | No Tag |
| Physical Appearance | Lyophilized powder |
| Storage Buffer | PBS, pH7.4 |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage | ·12 months from date of receipt, lyophilized powder stored at -20 to -80℃. |
| Reference | 1. J Biol Chem. 1996 Jun 21;271(25):15292-7. |
Background
Basic fibroblast growth factor (bFGF), also known as FGF2, is a member of the fibroblast growth factor (FGF) family. It is a highly specific chemotactic and mitogenic factor for many cell types, appears to be involved in remodeling damaged tissue, such as ulcer healing, vascular repair, traumatic brain injury (TBI). bFGF is a critical component of human embryonic stem cell culture medium. In addition, bFGF protein is a heparin-binding cationic protein involved in a variety of pathological conditions including angiogenesis and solid tumour growth. Thus, bFGF is regarded as a target for cancers chemopreventive and therapeutic strategies.
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Bioactivity
SDS-PAGE
2μg (R: reducing condition, N: non-reducing condition).
SEC-HPLC
>95% as determined by SEC-HPLC.
RP-HPLC
>95% as determined by RP-HPLC.
