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FGF-basic Protein, Chicken

FGF-basic Protein, Chicken

Catalog Number: UA040550 Brand: UA BIOSCIENCE
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$200 USD
$200 USD
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Product Details

Product Specification


Species Chicken
Synonyms Fibroblast growth factor 2, FGF-2, Basic fibroblast growth factor (bFGF), Heparin-binding growth factor 2 (HBGF-2)
Accession P48800
Amino Acid Sequence

Pro13-Ser158
Expression System E.coli
Molecular Weight

14-15 kDa (Reducing)
Purity >95% by SDS-PAGE & HPLC.
Endotoxin <0.1EU/μg
Conjugation Unconjugated
Tag No Tag
Physical Appearance Lyophilized powder
Storage Buffer

PBS, pH7.4
Reconstitution

Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
Stability & Storage

·12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
·3 months, -20 to -80℃ under sterile conditions after reconstitution.
·1 week, 2 to 8℃ under sterile conditions after reconstitution.
·Please avoid repeated freeze-thaw cycles.
Reference

iScience. 2022 Sep 3;25(10):105054.
J Bone Miner Res. 1994 Mar;9(3):431-9.

Background

Basic fibroblast growth factor (bFGF), also known as FGF-2 or FGF-β, is a member of the fibroblast growth factor family that regulates essential biological functions including cell proliferation, survival, migration, and differentiation. The FGF family comprises 18-22 members grouped into subfamilies based on sequence homology, with FGF-2 binding to and signaling through all four FGF receptors (FGFR1-4), transmembrane receptors with intracellular tyrosine kinase activity. Upon activation, FGFRs recruit SH2 or PTB domain-containing signaling proteins, leading to activation of downstream pathways including RAS/MAP kinase, PI3 kinase/Akt, and PLCγ.

The industrial applications of chicken bFGF have expanded dramatically with the emergence of cellular agriculture and cultivated meat production. Recombinant chicken/duck FGF-2 protein (145 aa) is specifically developed for species-specific avian cellular agriculture protocols, serving as a critical medium component for cultivating chicken cells for food applications.

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Bioactivity

Measured in a cell proliferation assay using Balb/c 3T3 mouse fibroblast cells. The EC50 for this effect is 0.030-0.061 ng/ml.

SDS-PAGE

2μg (R: reducing condition, N: non-reducing condition).

SEC-HPLC

>95% as determined by SEC-HPLC.