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Ferritin Light Chain Recombinant Rabbit mAb (S-1879-19)

Ferritin Light Chain Recombinant Rabbit mAb (S-1879-19)

Catalog Number: S0B1292 Application: WB,IHC-P Reactivity: Human Conjugation: Unconjugated Brand: Starter
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Regular price $130.00 SGD
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Product Details

Product Specification


Host Rabbit
Antigen Ferritin Light Chain
Synonyms Ferritin L subunit; FTL
Immunogen Recombinant Protein
Location Cytoplasm
Accession P02792
Clone Number S-1879-19
Antibody Type Recombinant mAb
Isotype IgG
Application WB, IHC-P
Reactivity Hu
Positive Sample HeLa, HepG2
Purification Protein A
Concentration 0.5 mg/ml
Conjugation Unconjugated
Physical Appearance Liquid
Storage Buffer PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
Stability & Storage

12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution


application dilution species
WB 1:1000 Hu
IHC-P 1:4000 Hu

Background

Ferritin Light Chain (FTL) is a critical component of the ferritin complex, which is essential for iron homeostasis. It stores iron in a soluble, non-toxic form and facilitates its delivery to cells. Structurally, FTL, along with heavy subunits, forms a spherical shell that allows iron entry through pores. Mutations in the C-terminus can disrupt pore formation, impacting iron accumulation. FTL plays a significant role in kidney development by mediating iron uptake in capsule cells, which is crucial for organogenesis.

Picture

Western Blot

WB result of Ferritin Light Chain Recombinant Rabbit mAb
Primary antibody: Ferritin Light Chain Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 20 kDa
Observed MW: 22 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human colon. Anti-Ferritin Light Chain antibody was used at 1/4000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human liver. Anti-Ferritin Light Chain antibody was used at 1/4000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human bladder cancer. Anti-Ferritin Light Chain antibody was used at 1/4000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-Ferritin Light Chain antibody was used at 1/4000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-Ferritin Light Chain antibody was used at 1/4000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

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