WB result of FCER1A/FcεRIα Recombinant Rabbit mAb
Primary antibody: FCER1A/FcεRIα Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HEK-293 whole cell lysate 20 µg
Lane 2: KU812 whole cell lysate 20 µg
Negative control: HEK-293 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 30 kDa
Observed MW: 48 kDa
FCER1A/FcεRIα Recombinant Rabbit mAb (S-1697-174)
FCER1A/FcεRIα Recombinant Rabbit mAb (S-1697-174)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | FCER1A/FcεRIα |
| Synonyms | High affinity immunoglobulin epsilon receptor subunit alpha; Fc-epsilon RI-alpha (FcERI); IgE Fc receptor subunit alpha; FCE1A |
| Immunogen | Recombinant Protein |
| Location | Cell membrane |
| Accession | P12319 |
| Clone Number | S-1697-174 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu |
| Positive Sample | KU812 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
| IHC-P | 1:50 | Hu |
| ICC | 1:500 | Hu |
Background
FcεRIα (Fc epsilon receptor I alpha) is a type I transmembrane glycoprotein encoded by the FCER1A gene, which belongs to the immunoglobulin gene superfamily. It is a critical component of the high-affinity IgE receptor (FcεRI) complex, responsible for binding the Fc region of IgE antibodies with high affinity. This receptor complex is primarily expressed on mast cells and basophils, and to a lesser extent on some monocytes, Langerhans cells, dendritic cells, and eosinophils from allergic donors. Upon allergen binding to IgE, FcεRIα triggers cellular activation, leading to the release of mediators such as histamine and cytokines, which drive allergic responses and contribute to anti-parasite immunity.
Picture
Picture
Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human tonsil. Anti-FCER1A/FcεRIα antibody was used at 1/50 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-FCER1A/FcεRIα antibody was used at 1/50 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-FCER1A/FcεRIα antibody was used at 1/50 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human prostatic cancer. Anti-FCER1A/FcεRIα antibody was used at 1/50 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human renal clear cell carcinoma. Anti-FCER1A/FcεRIα antibody was used at 1/50 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in KU812 cells (top panel) and negative staining in 293T cells (below panel). Anti- FCER1A/FcεRIα antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
