Fc γ RIIIb/CD16b(NA1) His Tag Protein, Human
Fc γ RIIIb/CD16b(NA1) His Tag Protein, Human
Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | Fc γ RIIIb, CD16b(NA1);Low affinity immunoglobulin gamma Fc region receptor III-B, Fc-gamma RIII-beta, FcR-10, IgG Fc receptor III-1, FCG3, FCGR3, CD16b and FCGR3B. |
| Accession | O75015 |
| Amino Acid Sequence | Gly17-Ser200(NA1), with C-terminal His |
| Expression System | HEK293 |
| Molecular Weight | 33-40 kDa(Reducing) |
| Purity | ＞95%, by SDS-PAGE under reducing conditions |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | His Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | PBS, pH7.4, 5% trehalose |
| Reconstitution | Reconstitute at less than 1 mg/mL according to the size in ultrapure water after rapid centrifugation . |
| Stability & Storage | · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
Background
IgG Fc receptor (Fc γ R) is a member of the Ig superfamily, which plays a role in activating or inhibiting immune response. Human Fc γ Rs is identified as three types: RI (CD64), RII (CD32) and RIII (CD16). CD16 is a low-affinity Fc receptor, which has been identified as Fc receptor Fc γ RIII (a (CD16a) and Fc γ RIIIb (CD16b). These receptors bind to the Fc portion of the IgG antibody and are encoded by two different highly homologous genes in a cell type-specific manner. CD16b, also known as FCGR3b and FCG3b, is specifically expressed by neutrophils and stimulated eosinophils. CD16b is a low affinity receptor of immunoglobulin Fc γ region. CD16b binds composite or aggregated IgG and monomer IgG. CD16b could not mediate antibody-dependent cytotoxicity and phagocytosis.
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Bioactivity
SDS-PAGE
SPR
Anti-His antibody Immobilized on CM5 Chip captured Fc γ RIIIb/CD16b(NA1) His Tag, Human (Cat. No. UA020006), can bind Rituximab with an affinity constant of 1.93 μM as determined in SPR assay.
