WB result of FBXL12 Recombinant Rabbit mAb
Primary antibody: FBXL12 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: U-937 whole cell lysate 20 µg
Lane 3: Hep G2 whole cell lysate 20 µg
Lane 4: Ramos whole cell lysate 20 µg
Lane 5: HEK-293 whole cell lysate 20 µg
Lane 6: Caco-2 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 37 kDa
Observed MW: 33, 37 kDa
FBXL12 Recombinant Rabbit mAb (S-5276-37)
FBXL12 Recombinant Rabbit mAb (S-5276-37)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | FBXL12 |
| Synonyms | F-box/LRR-repeat protein 12; F-box and leucine-rich repeat protein 12; F-box protein FBL12; FBL12 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm |
| Accession | Q9NXK8 |
| Clone Number | S-5276-37 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HeLa, U-937, Hep G2, Ramos, HEK-293, Caco-2, NIH/3T3, mouse kidney, rat kidney, rat liver |
| Predicted Reactivity | Hm |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| IHC-P | 1:400 | Hu, Ms, Rt |
| ICC | 1:2000 | Hu |
Background
FBXL12 (F-box and leucine-rich repeat protein 12, also known as FBL12) is a substrate-recognition subunit of SCF-type E3 ubiquitin ligases; through its F-box domain it binds the SKP1–CUL1 complex and recruits specific substrates for polyubiquitination and proteasomal degradation. Its better-characterized substrates include calcium/calmodulin-dependent protein kinase I (CAMK1) and members of the ALDH3 family: FBXL12 mediates CAMK1 degradation, thereby interfering with cyclin D1/CDK4 complex assembly, causing G1 arrest in lung epithelial cells, and regulating T cell differentiation in a cell-autonomous manner. During placental development, FBXL12 downregulates ALDH3 activity by mediating its ubiquitination and degradation, thereby initiating the differentiation program of trophoblast stem cells; Fbxl12-deficient mice die during embryogenesis or perinatally due to impaired formation of the placental junctional zone. In addition, recent studies have revealed that FBXL12 also targets CHK1-phosphorylated FANCD2 for proteasomal degradation, thereby regulating replication fork restart and the replication stress response, and this FBXL12–FANCD2 axis is often overexpressed in highly proliferative tumors. Mouse genetic data show that Fbxl12 knockout leads to partial postnatal lethality, growth retardation, and abnormal placental vascular morphology.
Picture
Picture
Western Blot
WB result of FBXL12 Recombinant Rabbit mAb
Primary antibody: FBXL12 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse kidney lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 37 kDa
Observed MW: 33, 37 kDa
WB result of FBXL12 Recombinant Rabbit mAb
Primary antibody: FBXL12 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat kidney lysate 20 µg
Lane 2: rat liver lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 37 kDa
Observed MW: 33, 37 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human kidney. Anti-FBXL12 antibody was used at 1/400 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human prostate. Anti-FBXL12 antibody was used at 1/400 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-FBXL12 antibody was used at 1/400 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-FBXL12 antibody was used at 1/400 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse kidney. Anti-FBXL12 antibody was used at 1/400 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat liver. Anti-FBXL12 antibody was used at 1/400 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in HEK293 cells. Anti-FBXL12 antibody was used at 1/2000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
