ICC shows positive staining in A549 cells. Anti- FASN (Alexa Fluor® 594 Conjugate) antibody was used at 1/500 dilution (Red) and incubated overnight at 4°C. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
FASN Recombinant Rabbit mAb (Alexa Fluor® 594 Conjugate) (S-682-32)
FASN Recombinant Rabbit mAb (Alexa Fluor® 594 Conjugate) (S-682-32)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | FASN |
| Synonyms | Fatty acid synthase; FAS |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm |
| Accession | P49327 |
| Clone Number | S-682-32 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | ICC, IF |
| Reactivity | Hu, Ms, Rt |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Alexa Fluor® 594 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.09% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| ICC | 1:500 | Hu |
| IF | 1:500 | Hu, Ms, Rt |
Background
Fatty acid synthase is a multi-enzyme protein that catalyzes fatty acid synthesis. It is not a single enzyme but a whole enzymatic system composed of two identical 272 kDa multifunctional polypeptides, in which substrates are handed from one functional domain to the next. Its main function is to catalyze the synthesis of palmitate (C16:0, a long-chain saturated fatty acid) from acetyl-CoA and malonyl-CoA, in the presence of NADPH. FAS is upregulated in breast and gastric cancers, as well as being an indicator of poor prognosis, and so may be worthwhile as a chemotherapeutic target. FAS may also be involved in the production of an endogenous ligand for the nuclear receptor PPAR alpha, the target of the fibrate drugs for hyperlipidemia, and is being investigated as a possible drug target for treating the metabolic syndrome.
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Immunocytochemistry
Immunofluorescence
IF shows positive staining in paraffin-embedded human colon cancer. Anti- FASN (Alexa Fluor® 594 Conjugate) antibody was used at 1/500 dilution (Red) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded human breast cancer. Anti- FASN (Alexa Fluor® 594 Conjugate) antibody was used at 1/500 dilution (Red) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded mouse liver. Anti- FASN (Alexa Fluor® 594 Conjugate) antibody was used at 1/500 dilution (Red) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded rat liver. Anti- FASN (Alexa Fluor® 594 Conjugate) antibody was used at 1/500 dilution (Red) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
