WB result of FACL4 Rabbit pAb
Primary antibody: FACL4 Rabbit pAb at 1/1000 dilution
Lane 1: MCF7 whole cell lysate 20 µg
Lane 2: T-47D whole cell lysate 20 µg
Lane 3: HeLa whole cell lysate 20 µg
Lane 4: 293T whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 79 kDa
Observed MW: 70 kDa
FACL4 Rabbit Polyclonal Antibody
FACL4 Rabbit Polyclonal Antibody
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | FACL4 |
| Synonyms | Long-chain-fatty-acid--CoA ligase 4; Arachidonate--CoA ligase; Long-chain acyl-CoA synthetase 4 (LACS 4); ACS4; LACS4; ACSL4 |
| Immunogen | Synthetic Peptide |
| Location | Cell membrane, Endoplasmic reticulum membrane, Mitochondrion outer membrane |
| Accession | O60488 |
| Antibody Type | Polyclonal antibody |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu |
| Positive Sample | MCF7, T-47D, HeLa, 293T |
| Purification | Immunogen Affinity |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
| IHC-P | 1:250 | Hu |
Background
FACL4 (Fatty Acid-CoA Ligase 4), also known as ACSL4, is an enzyme that belongs to the long-chain fatty-acid-CoA ligase family, encoded by the ACSL4 gene. This enzyme plays a crucial role in lipid metabolism by converting free long-chain fatty acids into fatty acyl-CoA esters, which are involved in various metabolic pathways including β-oxidation, glycerolipid synthesis, and protein acylation. FACL4 is unique in its preference for arachidonic acid (AA) as a substrate, and it helps regulate the levels of free AA in cells, which is known to induce apoptosis. The protein is highly expressed in tissues such as the brain, testis, ovary, spleen, and adrenal cortex, and its absence may contribute to conditions like intellectual disability or Alport syndrome. Additionally, FACL4 has been implicated in cancer development, with studies showing its upregulation in colon adenocarcinoma and hepatocellular carcinoma, suggesting its potential involvement in tumorigenesis.
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Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-FACL4 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver. Anti-FACL4 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human skeletal muscle. Anti-FACL4 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-FACL4 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
